Cyprinidae: Moenkhausia Pittieri Poptella

Saturday 21 July 2012

Moenkhausia Pittieri Poptella

Mitotic chromosome preparations were obtained from kidney and gill cells using the air-drying technique described by Foresti et al. (1993). Individual relative DNA content was determined according to the technique described by Gold and Price (1985) with some minor modifications. Blood was collected by caudal puncture and smeared near the frosted end on each of three slides. Blood smears from chicken, common carp, and rainbow trout served as standard references for DNA quantification. Absorbance values of fish nuclei from each slide were standardized as a percentage of the mean absorbance value of the three controls. To express DNA amount in picograms